Airway Microbiome in Asthma: Relationships to Asthma Phenotype and Inhaled Corticosteroid Treatment

Exclusion Criteria

• •Asthmatic:
• •Presence of lung disease other than asthma.
• •Use of \> 10 doses of nasal corticosteroids in the previous 3 months.
• •Presence of significant medical illness or other chronic diseases whose treatment could affect the clinical features measured, responses to the therapies to be given in this study, or risks of participating in the study.
• •History of atrial or ventricular tachyarrhythmia.
• •Changes suggestive of cardiac ischemia on ECG at baseline.
• •History of upper respiratory infection, sinusitis, bronchitis, or antibiotic use in the previous 3 months.
• •History of chronic sinus disease.
• •Smoking \> 5 pack-years, or within the past year
• •History of long-term controller medication use for asthma (inhaled or oral corticosteroid, leukotriene pathway antagonist, cromolyn, or theophylline within the preceding 6 months.
• •History of bleeding disorder.
• •Reduced creatinine clearance.
• •Inability, in the opinion of the Study Investigator, to coordinate use of inhaler or otherwise comply with medication regimens.
• •Contraindication to bronchoscopy on history or examination.
• •Healthy Control:
• •History of chronic respiratory disease including asthma.
• •Presence of significant medical illness or other chronic diseases whose treatment could affect the clinical features measured, responses to the therapies to be given in this study, or risks of participating in the study.
• •History of atrial or ventricular tachyarrhythmia.
• •Changes suggestive of cardiac ischemia on ECG at baseline.
• •History of upper respiratory infection, sinusitis, bronchitis, or antibiotic use in the previous 3 months.
• •Methacholine PC20 \< 16 mg/ml or FEV1 improvement ≥ 12% in response to albuterol.
• •History of chronic sinus disease
• •Smoking \> 5 pack-years, or within the past year
• •Use of \> 10 doses of a nasal corticosteroid preparation in the previous 3 months
• •FEV1 or FVC \< 80% predicted.
• •History of bleeding disorder.
• •Reduced creatinine clearance.
• •Contraindication to bronchoscopy on history or examination.