Effects of Electroacupuncture at NP82 and SP15 on Bowel Motility in Healthy Subjects

Background and Rationale: Bowel motility is a fundamental physiological function regulated by the autonomic nervous system. This study explores the potential of electroacupuncture (EA) to modulate this activity using a unique acupoint combination: Dai Hoanh (SP15), a classic point known for regulating the Spleen and Stomach, and Thong Tien (NP82), a newly proposed "extra point" specifically indicated for treating paralysis-related constipation in traditional literature. The study aims to provide the first modern scientific evidence for the effectiveness of NP82 in stimulating bowel motility. Methodology: The study follows a before-and-after intervention design involving 30 healthy volunteers. To ensure objective measurement, bowel sounds are recorded using the 3M™ Littmann® CORE Digital Stethoscope, which amplifies sounds and allows for computerized analysis, overcoming the subjectivity of traditional auscultation. The procedure consists of three phases: 1. Baseline Phase: Continuous 30-minute recording of bowel sounds to establish a physiological reference. 2. Intervention Phase: Simultaneous 20-minute recording during EA. Sterile needles (0.30×40mm) are inserted into NP82 and SP15 points until "De Qi" sensation is achieved. Stimulation utilizes a dense-disperse wave at a 10 Hz frequency, with intensity adjusted (0.1-1 mA) based on participant tolerance. 3. Post-intervention Phase: Continuous 30-minute recording after needle removal to evaluate sustained effects. Data Analysis: The primary quantitative variable is the Bowel Sound Interval (SSI), defined as the time between bowel sound events. Statistical analysis will be performed using Stata 17.0, employing Paired t-tests or Wilcoxon signed-rank tests to compare bowel sound frequencies across the three phases with a significance level of p \< 0.05.