Endotoxin Exposure to Examine the Role of Inflammation in Alcohol Use

Exclusion Criteria

• •1. Have a current (last 12 months) DSM-5 diagnosis of substance use disorder for any psychoactive substances other than alcohol and nicotine
• •2. Have a lifetime DSM-5 diagnosis of schizophrenia, bipolar disorder, or any psychotic disorder
• •3. Have current moderate to severe depression as indicated by a score of ≥ 21 on the Beck Depression Inventory - II (BDI-II)
• •4. Have current suicidal ideation or lifetime history of suicide attempt as reported on the Columbia-Suicide Severity Rating Scale (C-SSRS)
• •5. Have a positive urine screen for drugs other than cannabis;
• •6. Have clinically significant alcohol withdrawal symptoms as indicated by a score ≥ 10 on the Clinical Institute Withdrawal Assessment for Alcohol-Revised (CIWA-R)
• •7. Have an intense fear of needles or have had any adverse reactions to needle puncture
• •8. Be pregnant, nursing, or planning to become pregnant while taking part in the study
• •9. Have a body mass index (BMI) greater than 30
• •10. Have a medical condition that may interfere with safe study participation (e.g., unstable cardiac, renal, or liver disease, uncontrolled hypertension or diabetes, autoimmune or inflammatory disease)
• •11. Have clinically significant abnormal EKG
• •12. Have \> Grade 2 laboratory abnormalities, based on FDA Guidance Document "Toxicity Grading Scale for Healthy Adult and Adolescent Volunteers Enrolled in Preventive Vaccine Clinical Trials"
• •13. Have any other circumstances that, in the opinion of the investigators, compromises participant safety
• •14. To participate in the inflammatory challenge, participants must not show any of the following upon arrival to the inflammatory challenge study visit:
• •1. BrAC \> 0.000 g/dl
• •2. clinical withdrawal (CIWA-R) score ≥ 10
• •3. blood pressure ≤ 90/60 or ≥ 160/120
• •4. resting pulse ≤ 50 beats/minute or \> 100 beats/minute
• •5. temperature ≥ 99.5°F
• •6. recent (past 2 weeks) acute illness or vaccination
• •7. score of 10+ on Physical Sickness Symptoms Assessment