Association Between Training Load and Lactate and Other Metabolites Analyzed by Metabolomic and Proteomic Techniques

The study hypothesizes that the use of minimally invasive microsampling systems, and subsequent application of metabolomics and proteomics, will allow the detection of differences in the levels of lactate and other metabolites and proteins produced by the greater energy demand of the musculoskeletal system after a single collective training on the court, in federated basketball players. In addition, lactate levels will be correlated with the subjective sensation of perceived exertion. The main objective of the study is to apply metabolomics techniques to analyze lactate levels in capillary blood samples collected by a dried blood spot (DBS) microsampling device, and to study their correlation with the subjective sensation of perceived effort in federated basketball players before and after performing a single collective training session on the court. The secondary objectives of the study are to measure the change in lactate levels in capillary blood samples collected by a DBS device, and in saliva samples collected by a collector, before and after performing a single collective training on the court. In addition, in these samples, the change in the levels of other metabolomic and proteomic markers related to energy, lipid and amino acid metabolism will be measured. The correlation between salivary and blood lactate levels will also be studied; subjective sensation of perceived exertion and salivary lactate levels; the subjective sensation of perceived exertion and the levels of other metabolomic and proteomic markers will be also studied. A single-group quasi-experimental (or pre-post) study will be carried out on 70 basketball players between the ages of 18 and 40. Each participant will attend 2 visits to the sports facilities of their basketball club: * A recruitment and pre-selection visit (to check the eligibility criteria and sign the informed consent). If the inclusion criteria are met, will be scheduled to: * A single study visit on the same day agreed for training. The main variable of the study is the correlation between lactate levels, measured in capillary blood pre- and post-training, and the subjective sensation of perceived effort.