Effect of Total Sleep Deprivation on Vascular Function

Exclusion Criteria

• •Age \<20 or 31 to 49 or \>60 years
• •Body mass index ≥30 kg/m\^2 because obesity may affect vascular function
• •Use of medication that may affect vascular measures
• •Hormone replacement therapy or hormonal contraceptives within past year
• •Use of tobacco products (chewing tobacco, traditional or e-cigarettes) because they may influence vascular function
• •Being perimenopausal, pregnant or lactating because may influence vascular function
• •Being a shift worker because habitual sleep deprivation and altered circadian rhythm may influence vascular function.
• •Not having an "intermediate" chronotype based on the Morningness-Eveningness Questionnaire (MEQ; score \<31 or \>69) because this would influence the effect of overnight sleep deprivation on vascular function.
• •Sleep complaints based on the Pittsburgh Sleep Quality Index (PSQI; score \>5) because this would influence the effect of overnight sleep deprivation on vascular function.