Experimental Design: Cross-sectional study, with prospective inclusions, single center.
Investigations:
* Realization of bronchoscopy according to the indications (with or without bronchoalveolar lavage with or without bronchial biopsy, participation in research that do not modify patient's care)
* samples are sent to the laboratory, centrifuged and stored at -80°C until they were assessed.
* samples were used to specific analysis in this study:
* Study of qualitative expression of collagen IV and XIX by Reverse transcription polymerase chain reaction (RT-PCR) and Western Blot
* Study of quantitative variations of these collagens by ELISA
Statistical analysis planification:
* description of the data (mean and standard deviation),
* comparison of different groups (non-pathological vs pulmonary disease) by Student's t test, Wilcoxon, Chi2 or Fisher test.
Expected results and prospects:
The investigators should be able to demonstrate the presence of collagen IV and/or collagen XIX or different fragments in biological samples. The investigators would then be able to show variation in expression of these collagens according to different pathological situations. In the longer term, the objective is to validate these collagens as a markers of pulmonary pathologies or as therapeutic agents.
