Lipoprotein Lipase Enzyme Activity Assay Validation and Clinical Assessment

Exclusion Criteria

• •1. Past history of LPL deficiency
• •2. Hypercholesterolemia (Cholesterol \>200, LDL \>160, HDL \<35) hypertriglyceridemia (TG\>150), any familial lipid disorder
• •3. Diabetes mellitus (type 1 or 2)
• •4. Uncontrolled hypertension SBP\>140, DBP\>90
• •5. History of hemorrhagic stroke
• •6. Current pregnancy
• •7. History of major surgery, invasive procedure or trauma within the last 30 days, or planned major surgery within 30 days after participating in the study
• •8. Exposure to heparin (unfractionated heparin or low molecular weight heparin) within 30 days prior to screening
• •9. History of allergy to heparin
• •10. History of heparin-induced thrombocytopenia
• •11. Current smoking
• •12. Active ulcerative or angiodysplastic GI diseases
• •13. Thrombocytopenia or platelet disorders (Platelet count \<100,000/UL)
• •14. Major health issues which may affect the safety of study subject, including but not limited to:
• •* History of chronic kidney disease (eGFR\<60)
• •* Long term use of medications which alter lipid metabolism
• •* History of hepatic disease (ALT or AST more than 2 times the upper limit of normal)
• •* History of clotting disorders, any type of coagulation factor deficiency, or thrombocytopenia
• •* Chronic use of ASA, anticoagulants, platelet inhibitors
• •* Use of NSAIDS for more than 2 weeks prior to screening
• •* Anemia (hemoglobin \<13 g/dL)