Background:
Coastal communities in Sayung District, Demak Regency, face recurrent tidal flooding (rob) that causes continuous environmental and economic pressure. From a psychoneuroimmunological perspective, chronic psychosocial stress triggers systemic low-grade inflammation. Biomarkers such as MLR, NLR, and PLR serve as accessible systemic inflammatory indices that correlate with mood disorders and stress severity. Mindfulness body scan training aims to improve interoceptive awareness, emotional regulation, and parasympathetic activity, thereby reducing stress reactivity and systemic inflammation.
Study Design and Objectives:
This is an interventional pre-post experimental study conducted at the Sayung 1 Public Health Center (Puskesmas Sayung 1), Demak. The study evaluates changes in systemic inflammatory markers and psychological parameters pre- and post-intervention.
Intervention:
Participants will participate in a structured mindfulness body scan program over an 8-day period. The body scan protocol guides participants to direct attention sequentially to various body regions without judgment to enhance somatic awareness and stress mitigation.
Assessments:
1. Laboratory Testing: Complete blood counts to determine monocyte, neutrophil, platelet, and lymphocyte ratios (MLR, NLR, PLR) pre- and post-intervention.
2. Psychometric Evaluations: Assessment of psychological symptoms using validated tools, including:
* Beck Depression Inventory (BDI) \& Patient Health Questionnaire-9 (PHQ-9)
* Depression, Anxiety, and Stress Scale-21 (DASS-21)
* Impact of Event Scale - Revised (IES-R) \& PTSD Checklist-5 (PCL-5)
* Pittsburgh Sleep Quality Index (PSQI)
* Connor-Davidson Resilience Scale (CD-RISC)
* Spiritual Wellbeing Scale (SWBS)
* Fatigue Assessment Scale (FAS)
* WHO Quality of Life (WHOQOL)
* Perceived Social Performance (PSP)
Data Analysis:
Pre- and post-intervention data will be analyzed using paired statistical tests and multivariate regression to determine changes in inflammatory markers and psychological scores, as well as the correlation between systemic inflammation changes and psychological recovery.
