Evaluating the Effect of Nicotine on Eye Movements and Related Behaviors in Electronic Cigarette Users, NICS-EYES Trial

PRIMARY OBJECTIVE: I. To determine the between-session reliability of oculomotor biomarkers during standardized nicotine administration in daily e-cigarette users. SECONDARY OBJECTIVES: I. To evaluate the association between oculomotor (OM) responses to nicotine and behavioral economic indices of reinforcing value. II. To assess the relationship between oculomotor changes and nicotine withdrawal relief. OUTLINE: Participants abstain from nicotine for at least 12 hours and caffeine products for at least 2 hours, as well as obtain sleep for at least 5-6 hours before each study visit. Participants self-administer 5% nicotine non-mint menthol pods via an e-cigarette device using a guided standardized puffing routine over 5 minutes on visit one. At least 48 hours later, participants complete the same 5-minute guided puffing routine as in visit one and then use an e-cigarette as they normally would over 30 minutes at visit two. Participants also complete eye movement testing and undergo blood sample collection during each visit.