Age

18–any

Sex

ALL

Healthy volunteers

Not accepted

Primary Total Knee Arthroplasty (TKA) or Primary Total Knee Arthroplasty (TKA) with Prosthetic Joint Infection (PJI)
18 years
Able to provide informed consent

Incomplete irrigation documentation
Patients with severe immunosuppression (example: chemotherapy, neutropenia, Human Immunodeficiency Virus \[HIV\], steroid use)
Positive fungal, parasitic, or mycobacterial cultures
Methylene Blue allergy
Patients on Monoamine Oxidase (MAO) inhibitors, Selective Serotonin Reuptake Inhibitors (SSRI), Selective Norepinephrine Reuptake Inhibitors (SNRI), and/or Tricyclic Antidepressants (TCA)
History of methemoglobinemia
Glucose-6-Phosphate (G6P) Enzyme Deficiency
Chronic Kidney disease or Glomerular Filtration Rate (GFR) less than 30mL/min
Prisoners
Pregnant Woman

Methylene Blue in Total Knee Arthroplasty (TKA)