An Exploratory Study on Microbial Biomarkers Associated With the Efficacy of Neoadjuvant Therapy

Exclusion Criteria

• •Physically unfit to undergo neoadjuvant chemotherapy
• •Local recurrence or distant metastasis occurring within 1 month after surgery
• •Impaired vital organ function, including but not limited to: Heart failure (NYHA Class III-IV); Myocardial infarction within the past 6 months; Severe arrhythmia; Respiratory failure
• •Presence of another primary malignancy diagnosed within the past 5 years
• •Pregnant or breastfeeding
• •Inability to complete follow-up
• •Active infection
• •Severe constipation or sudden changes in bowel habits within the past 3 months
• •Significant changes in dietary habits (e.g., meat-vegetable balance, meal timing, or frequency) within the past 3 months
• •Medication history within the past 3 months including:Use of NSAIDs, immunosuppressants, antibiotics, traditional Chinese medicine, probiotics, or corticosteroids for ≥1 week; Use of proton pump inhibitors (PPIs) for more than 1 week within 1 month prior to sampling
• •Refusal to sign the informed consent form