Type of the study: Prospective, comparative, split-face study. - Study Setting: The study will be done in Assiut University Hospital ,dermatology department ,after obtaining approval from ethics committee of Faculty of Medicine, Assiut University in Assiut, Egypt.
Sample Size Calculation:
Based on determining the main outcome variable, the estimated minimum required sample size is 42 cases (21 in each group). Sample size was calculated using G\*Power software version 3.1.9.7, based on the following assumptions: The main outcome is the difference in mean Goodman and Baron quantitative scar score between two independent groups of patients: those treated with Subcision + PRP and those treated with Subcision + Fractional CO₂.
Based on clinical experience and after reviewing the literature, a large difference was assumed.
The main statistical test is a one-tailed independent t-test to detect the difference between two means.
Effect size = 0.8 Alpha = 0.05 Power = 0.80
All patients will undergo subcision on both sides of the face, patients will be randomly divided into two groups:
group I (21 patients)-subcision combined with platelet rich plasma injection at right side of the face and subcision alone at the other side.
group II (21 patients)-subcision combined with fractional CO2 at right side of the face and subcision alone at the other side .
Each side will be randomly selected by choosing a sealed opaque envelope containing a card labeled with either laser or PRP representing the right split face side treatments.
Each patient will undergo three sessions with one month interval in between. Patient preparation
1. Each patient's entire face will be disinfected with alcohol and cleaned with saline.
2. Anesthesia will be injected subcutaneously with 2% lidocaine
3. A sharp needle inserted at a shallow angle, with the blade facing upward, at the periphery of the scarred area to create a pathway to facilitate the entry of the blunt cannula.
4. Subcision will be performed with an 18-gauge blunt cannula introduced at a 30° angle into the mid to deep dermis, then moved back and forth in a fan-like motion under the scar to release fibrous bands at the dermal or deep dermal, subcutaneous plane until the sound of bands breaking lost, and no resistance felt (endpoint).
5. To establish hemostasis, firm pressure should be applied for at least 5 min.
6. After subcision, the face should be cleaned with alcohol swabs and then sterile saline.
PRP side (group I): Right side of the face will be injected with PRP and the other by saline.
Fractional side (group II): Right side of the face will be treated with a fractional CO2 laser (10,600 nm) using post acne scar mode (DAESHIN ENTERPRISE CO2, LTD. (D.S.E) 105,207,Digital ro,Guro-gu, Seol, Korea).
Post procedure care All patients should be instructed to avoid sunlight exposure and to use sunscreen (SPF ≥ 50).
Cold compresses in first 24h-48h to avoid edema and ecchymosis. Use topical antibiotic ointment as mupirocin or fusidic acid. Analgesics as paracetamol to decrease the pain.
-Research outcome measures:
1. Primary (main):
Goodman \& Baron qualitative \& quantitative grading systems.
2. Secondary (subsidiary):
Photography, quartile grading scale. dermoscopic evaluation (erythema, pigmentation, photodamage, follicular density) Data entry and analysis will be carried out using SPSS version 21. Descriptive statistics will be performed with frequency and percentages for categorical variables. Mean and standard deviation for normally distributed numerical variables, while median and interquartile measures for non-normally distributed numerical. Then analytic statistics will be done as the chi-square test for comparing independent categorical variables, and McNemar's test. If the data is normally distributed independent t-test, paired t-test, ANOVA, and Pearsons correlations tests will be used. If the data is not normally distributed Mann-Whitney, Kruskal-Wallis, Willcoxon signed-rank, Friedman, and Spearmans correlation tests will be used. Values will be considered significant when P values equal or less than 0.05.
