Feasibility Study of Tissue and Blood Collection in Oncogene-addicted and Neoadjuvantly Treated Non Small Cell Lung Cancer

Rationale Benchmarking the proportion of patients who are able to provide paired samples of blood and viable tumour tissue is important in oncogene-addicted metastatic NSCLC patients as investigators have now entered the era of genotype-guided post-progression targeted therapies. In the early-stage operable NSCLC context, benchmarking the feasibility of paired samples is of interest as neoadjuvant CPI-based therapy has recently emerged as a new standard of care strategy. As such, investigators have defined the following cohorts within our study: Cohort 1: Oncogene-addicted NSCLC, due to commence new line of targeted therapy * Sub-cohort 1A: treatment naïve, oncogene-addicted NSCLC * Sub-cohort 1B: pre-treated, oncogene-addicted NSCLC, received prior targeted therapy * Sub-cohort 1C: pre-treated, oncogene-addicted NSCLC, no prior targeted therapy (can have received chemotherapy/CPI/chemo-CPI) Cohort 2: Early-stage operable NSCLC undergoing neoadjuvant CPI therapy Primary aim To estimate the feasibility of collecting paired samples of blood and viable tissue in patients with: * Oncogene-addicted metastatic NSCLC commencing new line of targeted therapy at progression (Cohort 1), and * Early-stage operable NSCLC undergoing neoadjuvant CPI-based therapy (Cohort 2) Viable tissue is defined by the presence of viable tumour cells. Viable tumour cells are defined by those with well-preserved architectural and cytological features, in line with the latest recommendation from the International Association for the Study of Lung Cancer (IASLC), evaluated by a specialist pulmonary pathologist using a haematoxylin \& eosin (H\&E) stained slide. Secondary aims To estimate the feasibility of obtaining viable tissue samples at: * Baseline (sub-cohort 1B) * PD (Cohort 1) * Surgery (Cohort 2) Exploratory aims * To identify genes/proteins associated with disease progression on targeted therapy (Cohort 1) * To measure levels of tumour cell-recognising antibodies in patients on targeted therapy (Cohort 1) * To identify genes/proteins associated with tumour regression (Cohort 2)