Characterizing the Host Response to Leptospirosis for Better Diagnosis and Prognosis - NIHFI

Inclusion Criteria

• •For all participants :
• •Be able to consent,
• •Have received information and given written consent,
• •Be covered by a social security plan. For groups 1 \& 2: febrile individuals
• •Have suspected leptospirosis associated with fever (axillary temperature over 38°C) and a clinical picture of suspected leptospirosis (myalgia, headache...). After inclusion, febrile participants will be divided into 2 groups when the diagnosis of leptospirosis is confirmed. The 2 groups are defined as follows:
• •Group 1: leptospirosis
• •\- Individual with leptospirosis confirmed by PCR, MAT, or isolation (2013 Center For Disease Control and Prevention (CDC) laboratory criteria for a confirmed diagnosis).
• •Group 2: MFA, with absence of leptospirosis
• •All individuals in Group 2 will have tests as part of their MFA diagnosis, depending on their symptomatology:
• •Other acute bacterial zoonotic infection: Infection confirmed by paired serology or double PCR (2 targets).
• •Acute arboviral infection: dengue, chikungunya or Zika virus confirmed by CDC Trioplex PCR in a patient with consistent serology or positive 2nd target PCR.
• •Acute respiratory viral infection: Viral agent confirmed by MFA multiplex PCR. For group 3: healthy individuals
• •Apyretic individuals, no symptoms of infection or inflammatory disease in the last 28 days.