Comparison of Effectiveness of Disinfectants

The study design is a comparative study which was done to compare the efficacy of chemical disinfection agents on decontaminating the gutta percha cones . In this study, 124 standardized size-80 gutta-percha (GP) cones (Dentsply, Germany) were used to assess the efficacy of chemical disinfectants. The cones were first sterilized using ethylene oxide to ensure baseline sterility. Following sterilization, 124 cones were allocated into two experimental groups (n = 62 each) for contamination. group A . gp were immersed in 20ml of Enterococcus faecalis solution group B . gp were immersed in 20 ml of human saliva in patients undergoing root canal therapy samples were incubated at 37°C for 72 hours to ensure uniform and thorough contamination after incubation period cones were dried and divide into subgroups based on disinfectant agent applied . The cones were immersed in disinfectant agent for 1 min and 10 min . Cones were dried again and immersed in brain heard infusion broth samples were again incubated at 37°C for 72 hours The presence of bacterial growth was determined by assessing turbidity in the broth, indicating viable microorganisms.