Specific Aim (SA) 3:
The objective of SA 3 is to examine the effectiveness of Native Spirit (NS) in the Salt River Pima Maricopa Indian Community (SRPMIC) and Pascua Yaqui Tribe (PYT) adolescents (grades 7-12) on preventing or decreasing substance use and strengthening cultural identity, self-esteem, and resilience. SA 3 will use a wait-list control design.
Procedure:
Step 1: Recruitment and Consent 1.1 Recruitment and consent process will occur December 2024-January 2025 for SRPMIC and July-September 2025 for PYT Cohort 1 and Jul7-September 2026 for PYT Cohort 2.
Step 2: Baseline Assessment - Time 1 (T1)
2.1 SRPMIC participants will take the T1 assessment in January 2025. Surveys will be conducted virtually on participant cell phones. The research team will also print out multiple hard copies of the survey for participants who do not have access to a cell phone. Participants will sit at individual desks that are spaced so they cannot see each others assessment answers to minimize confidentiality risks. PYT participants will take the T1 assessment in August 2025.
Who: Virtual surveys will be administered by the PI and research team. Where: T1 assessment will be completed at the research sites - the SRPMIC Way of Life Facility and the PYT Boys \& Girls Club.
How Long: T1 assessment will take 20-30 minutes to complete. How/What data: T1 assessment data will be collected virtually through REDCap. Duration: T1 assessment data will be completed in January 2025 for SRPMIC and August 2025 for PYT.
Step 3: Spring 2025 Intervention 3.1 SA3 uses a wait-list control design. The SRPMIC will be allocated to the intervention first. This is because the PI has worked with the SRPMIC for five years and the SRPMIC already has a developed intervention curriculum (the PI will submit a modification for review once curriculum is updated for 2025 intervention). This will allow the PI and the PYT to adapt the intervention. The intervention group (SRPMIC) will receive the intervention (NS program). Each intervention session is based on an Indigenous cultural value and cultural activity/practice that is specific to the SRPMIC.
Who: Intervention sessions will be facilitated by local cultural knowledge holders. At least one member of the research team will attend each session to ensure fidelity Where: Intervention sessions will be held at the research sites - the SRPMIC Way of Life Facility How Long: Each intervention session will last for 60-90 minutes, once a week for 10 weeks Duration: The Spring intervention will take place from January 2025 - May 2025
3.2 The research team will take candid pictures during the intervention that can be used for results dissemination. Any identifiable pictures with youth who do not have signed assent/consent forms will be immediately deleted.
Step 4: Follow-Up Assessment - Time 2 (T2) 4.1 SRPMIC participants will take the T2 assessment in May 2025. Surveys will be conducted virtually on participant cell phones. The research team will also print out multiple hard copies of the survey for participants who do not have access to a cell phone. Participants will sit at individual desks that are spaced so they cannot see each other's assessment answers to minimize confidentiality risks.
Who: Virtual surveys will be administered by the PI and research team. Where: T2 assessment will be completed at the research site - the SRPMIC Way of Life Facility How Long: T2 assessment will take 20-30 minutes to complete. How/What data: T2 assessment data will be collected virtually through REDCap. Duration: The T2 assessment will be completed in May 2025
Step 5: Spring Participant interviews
5.1 Participants who received the NS intervention in the Spring will be recruited to participate in the participant interviews based on their quantitative assessment scores. The research team will recruit participants who are interested in being interviewed. Each participant will complete an individual, 30-60-minute interview intended to identify personal impacts of participation in the NS program on self-esteem, resilience, cultural identity, and substance use. Interview questions are included in an attachment Who: Interviews will be conducted by the research assistants who have received training in interview procedures.
Where: Interviews will be held in private rooms at the research site - the SRPMIC Way of Life Facility How/What data: Interview data will be collected with a digital recorder. How Long: Each interview will last for 30-45 minutes Duration: Spring interviews will be completed between May-June 2025
Step 6: Fall 2025 Usual Programming
6.1 Both groups (SRPMIC and PYT) will receive control or "usual" programming. Usual programming includes a life skills program that focuses on financial wellness for teens, called "Money Matters" (Usual Practice). Participants will learn valuable financial life skills.
Who: Control sessions will be facilitated by the research site partner (after-school program employees) who typically implement their usual programming Where: Control sessions will be held at the research site - the SRPMIC Way of Life Facility and the PYT Boys \& Girls Clubs How Long: Each control session will last for 60-90 minutes, once a week for 10 weeks Duration: The Fall Usual Programming will take place from August 2025 - December 2025
Step 7: Follow-Up Assessment - Time 2 (T2) and Time 3 (T3)
7.1 SRPMIC participants will take the T3 assessment in December 2025 and PYT participants will take the T2 assessment also in December 2025. Surveys will be conducted virtually on participant cell phones. The research team will also print out multiple hard copies of the survey for participants who do not have access to a cell phone. Participants will sit at individual desks that are spaced so they cannot see each other's assessment answers to minimize confidentiality risks.
Who: Virtual surveys will be administered by the PI and research team. Where: T3 and T2 assessments will be completed at the research sites - the SRPMIC Way of Life Facility and the PYT Boys \& Girls Club How Long: T2 and T3 assessments will take 20-30 minutes to complete. How/What data: T2 and T3 assessment data will be collected virtually through REDCap.
Duration: The T2 and T3 assessments will be completed in December 2025
Step 8: Spring 2026 Intervention
8.1 The wait-list group (PYT Cohort 1) will receive the intervention (NS program). Each intervention session is based on an Indigenous cultural value and cultural activity/practice that is specific to the PYT. The PYT intervention curriculum will be developed between July 2025-December 2025. It will have a similar organizational structure.
Who: Intervention sessions will be facilitated by local cultural knowledge holders. At least one member of the research team will attend each session to ensure fidelity Where: Intervention sessions will be held at the research site - PYT Boys \& Girls Club How Long: Each intervention session will last for 60-90 minutes, once a week for 10 weeks Duration: The Spring 2026 Cohort 1 intervention will take place from January-March 2026.
8.2 The research team will take candid pictures during the intervention that can be used for results dissemination. Any identifiable pictures with youth who do not have signed assent/consent forms will be immediately deleted.
Step 9: Follow-Up Assessment - Time 3 (T3)
9.1 PYT Cohort 1 participants will take the T3 assessment in March 2026. Surveys will be conducted virtually on participant cell phones. The research team will also print out multiple hard copies of the survey for participants who do not have access to a cell phone. Participants will sit at individual desks that are spaced so they cannot see each other's assessment answers to minimize confidentiality risks.
Who: Virtual surveys will be administered by the PI and research team. Where: T3 assessment will be completed at the research site - the PYT Boys \& Girls Club How Long: T3 assessment will take 20-30 minutes to complete. How/What data: T3 assessment data will be collected virtually through Qualtrics.
Duration: T3 assessment will be completed in May 2026.
Step 10: Participant interviews
10.1 PYT Cohort 1 Participants who received the NS intervention in Spring 2026 will be recruited to participate in the participant interviews based on their quantitative assessment scores. The research team will recruit participants who are interested in being interviewed. Each participant will complete an individual, 30-60-minute interview intended to identify personal impacts of participation in the NS program on self-esteem, resilience, cultural identity, and substance use.
Who: Interviews will be conducted by the research assistants who have received training in interview procedures.
Where: Interviews will be held in private rooms at the research site - the PYT Boys \& Girls Club How/What data: Interview data will be collected with a digital recorder. How Long: Each interview will last for 30-45 minutes Duration: Spring interviews will be completed in March2026
Step 11: PYT Cohort 2 Baseline Assessment - Time 1 (T1)
11.1 PYT Cohort 2 participants will take the T1 assessment in September 2026. Surveys will be conducted virtually on participant cell phones. The research team will also print out multiple hard copies of the survey for participants who do not have access to a cell phone. Participants will sit at individual desks that are spaced so they cannot see each others assessment answers to minimize confidentiality risks.
Who: Virtual surveys will be administered by the PI and research team. Where: T1 assessment will be completed at the research site - the PYT Boys \& Girls Club.
How Long: T1 assessment will take 20-30 minutes to complete. How/What data: T1 assessment data will be collected virtually through REDCap. Duration: T1 assessment data will be completed in September 2026 for PYT Cohort 2.
Step 12: Fall 2026 Intervention
12.1 The wait-list group (PYT Cohort 2) will receive the intervention (NS program). Each intervention session is based on an Indigenous cultural value and cultural activity/practice that is specific to the PYT.
Who: Intervention sessions will be facilitated by local cultural knowledge holders. At least one member of the research team will attend each session to ensure fidelity Where: Intervention sessions will be held at the research site - PYT Boys \& Girls Club How Long: Each intervention session will last for 60-90 minutes, once a week for 10 weeks Duration: The Fall 2026 Cohort 2 intervention will take place from September-December 2026.
12.2 The research team will take candid pictures during the intervention that can be used for results dissemination. Any identifiable pictures with youth who do not have signed assent/consent forms will be immediately deleted.
Step 13: Follow-Up Assessment - Time 2 (T2)
13.1 PYT Cohort 2 participants will take the T2 assessment in December 2026. Surveys will be conducted virtually on participant cell phones. The research team will also print out multiple hard copies of the survey for participants who do not have access to a cell phone. Participants will sit at individual desks that are spaced so they cannot see each other's assessment answers to minimize confidentiality risks.
Who: Virtual surveys will be administered by the PI and research team. Where: T2 assessment will be completed at the research site - the PYT Boys \& Girls Club How Long: T2 assessment will take 20-30 minutes to complete. How/What data: T2 assessment data will be collected virtually through Qualtrics.
Duration: T2 assessment will be completed in December 2026.
Step 14: Participant interviews
10.1 PYT Cohort 2 Participants who received the NS intervention in Fall 2026 will be recruited to participate in the participant interviews based on their quantitative assessment scores. The research team will recruit participants who are interested in being interviewed. Each participant will complete an individual, 30-60-minute interview intended to identify personal impacts of participation in the NS program on self-esteem, resilience, cultural identity, and substance use.
Who: Interviews will be conducted by the research assistants who have received training in interview procedures.
Where: Interviews will be held in private rooms at the research site - the PYT Boys \& Girls Club How/What data: Interview data will be collected with a digital recorder. How Long: Each interview will last for 30-45 minutes Duration: Fall interviews will be completed in December 2026
