The data for this study were obtained from patients who presented for treatment at the Department of Orthodontics at Bezmialem Vakif University Faculty of Dentistry. All patients were treated with clear aligners (Invisalign®, Align Technology, CA, USA) at Bezmialem University Hospital. Sequential distalization (50%) was planned in the maxillary arch. For each aligner, a staging of 0.25 mm was determined. Distalization starts with the second molars, then the first molars after half of the second molar movement is completed, and finally the premolars. In the ClinCheck planning, 3 or 4 mm vertical rectangular attachments were placed from the second molar to the canine and were bevelled mesially to enhance the distalization forces. The patients were divided into two groups, those who were treated with sequential distalization with aligners supported by Class II elastics (group 1) or IZC miniscrews (group 2). In group 1, with the beginning of the movement of the upper first molars, Class II intermaxillary elastics were used from precision cuts of the upper canines to the buttons on the lower first molars. In group 2, IZC miniscrews were inserted in the IZC region between the upper molars in both sides before first molar movement started. The same intramaxillary elastics were used from precision cuts of the upper canines to the IZC miniscrew. The use of intra/intermaxillary elastics was started with the initiation of upper first molar movement to prevent anchorage loss and maxillary incisor proclination. Both groups were instructed to wear the elastics and aligners a minimum of 22 hours per day using a 10-day aligner wear protocol. Maxillary third molars were extracted before treatment.
Patients were scanned by iTero 5D Element intraoral scanner and radiographs were taken before treatment (T0) and after distalization of the second premolar (T1).
Cephalometric radiographs were obtained from all patients and these radiographs were used for two-dimensional measurements.Digital tracing and measurements of the cephalometric radiographs were performed using NemoCeph version 6.0 software. Angular dental measurements were performed between the long axis of the teeth and the sella-nasion (S-N) plane using Image J software. The long axis was determined as a line through the incisal edge and the root apex for the incisors, through the buccal cusp tip and the root apex for the premolars, and through the centroid and the furcation for the molars. Digital models were exported from OrthoCAD software as stereolithography (STL) files and imported into Geomagic Control X software for three-dimensional (3D) model comparison. All digital models were superimposed using the local best fit algorithm. A reference area in the palatal region was used for the model superimposition and 50 iteration points were defined in this area for the superimpositions.
After model superimposition, reference points of the teeth were selected on the two models to enable linear measurements. These points were the mesiobuccal cusp tips of the maxillary molars and the buccal cusp tips of the premolars. These reference points were marked on the T0 and T1 models and measurements were taken to observe changes on all three axis. In linear measurements,
1. X-axis represent buccolingual (transversal) differences (X+ in the buccal direction and X- in the palatal direction);
2. Y-axis represent mesiodistal (sagittal) differences (Y+ in the distal direction and Y- in the mesial direction); and
3. Z-axis represent vertical differences (Z+ indicating extrusion and Z- indicating intrusion).
For angular measurements, reference points and vectors were identified on 3D models. These points were the mesiobuccal and mesiopalatal cusp tips of the molars and the buccal and palatal cusp tips of the premolars. The angles between reference vectors were measured. Rotational movements were measured using the XY-axis, with positive values indicating mesiobuccal rotation and negative values indicating distobuccal rotation.
