Deep Phenotyping of Peripheral Blood Cells and Circulating Factors in Metabolic Diseases

Exclusion Criteria

• •1. Diabetes mellitus Typ 1
• •2. BMI \< 18.5 kg/m2
• •3. Transfusion of blood or major bleeding in the last six months
• •4. Anaemia with haemoglobin \< 9,0 g/dl
• •5. Chronic alcohol or drug abuse
• •6. Presence of any acute or chronic liver disease apart from non-alcoholic fatty liver disease (i.e. viral, autoimmune or alcoholic hepatitis, haemochromatosis, Morbus Wilson etc.)
• •7. Systemic infections (CRP \> 1 mg/dl)
• •8. Medications that affect blood glucose levels (e.g. antidiabetics \[except from the subjects forming the diabetes group\], steroids) in the last six months
• •9. Medications that affect coagulation (e.g. anticoagulants and antiplatelet agents) in the last six months
• •10. Medications that affect immune function (e.g. immunosuppressive drugs) in the last six months
• •11. Pregnancy or breastfeeding
• •12. Severe psychic disorders
• •13. Inability to follow the study protocol
• •14. Have any medical condition unsuitable for inclusion in the study, in the opinion of the investigator
• •1. Pacemaker
• •2. Artificial heart valve
• •3. Metal prosthesis
• •4. Implanted magnetic metal parts
• •5. Spirals
• •6. Fixed metal dental braces
• •7. Acupuncture needle
• •8. Insulin pumps
• •9. By MRI \> 3 Tesla: Tattoos, permanent eyeliner
• •10. Claustrophobia or any other condition, such as psychiatric disorder, that in the opinion of the investigator may prevent the participant from following and completing the protocol
• •11. Subject dimensions not allowing the performance of MRI