Safety and Tolerability of CVGBM in Adults With Newly Diagnosed MGMT-Unmethylated Glioblastoma or Astrocytoma

Exclusion Criteria

• •1. Hemoglobin \<10 g/dL (6.2 mmol/L)
• •2. White blood cell (WBC) count decrease (\<2.5 x109/L)
• •3. Absolute neutrophil count (ANC) decrease \<1.5 x109/L
• •4. Platelet count decrease \<75 x109/L
• •5. Bilirubin \>1.5 x upper limit of normal (ULN according to the performing lab's reference range), except for patients with Gilbert's syndrome
• •6. Alanine aminotransferase (ALT) \>3 x ULN
• •7. Aspartate aminotransferase (AST) \>3 x ULN
• •8. Gamma glutamyltransferase (GGT) \>2.5 x ULN
• •9. Serum creatinine increased \>1.5 x ULN
• •2. Tumor biopsy only without gross total or partial resection (i.e., ≥50% of tumor volume resected).
• •3. Any prior therapy for GBM (except surgery, radiotherapy with or without chemotherapy (e.g., temozolomide \[TMZ\]), TTFields, and steroids) including immunotherapy.
• •4. Patient on stable or decreasing steroid levels exceeding 10 mg/day prednisone (or equivalent doses of other steroids) during the last 3 days prior to enrollment. Expectation that the patient will need steroid doses \>10 mg/day prednisone or equivalent during the next 3 months. Note: Steroid treatment during the study will be allowed for treatment of cerebral edema or other life-threatening conditions.
• •5. Active human immunodeficiency virus (HIV) infection (ie, CD4 count below the normal range) or active Hepatitis B or C infection (i.e., detectable levels of Hepatitis B DNA or Hepatitis C RNA), or active infections requiring oral or intravenous antibiotics or that can cause a severe disease.
• •6. Clinically relevant autoimmune diseases that could impact the assessment of vaccine safety and efficacy (with the exception of clinically stable thyroid diseases under medication and vitiligo).
• •7. Immunosuppression, not related to prior treatment for malignancy. Any medical condition that requires chronic systemic immunosuppressive therapy including chronic corticosteroids (except physiologic maintenance/replacement doses), methotrexate, tacrolimus or any other immunosuppressive agents within 28 days of treatment start, including, but not limited, to organ transplant-related immunosuppression.
• •8. Patients with prior hematopoietic stem cell transplantation/prior organ allograft.
• •9. Any condition that in the judgment of the Investigator is likely to prevent compliance with study procedures.
• •10. Patients with impaired coagulation or any bleeding disorder in whom an intramuscular injection or blood draw is contraindicated.
• •11. History of myocarditis or pericarditis within the last 3 months or history of myocarditis or pericarditis following COVID-19 vaccination.
• •12. Previous mRNA vaccination (e.g., SARS-CoV2) or live attenuated vaccination within 1 month prior to study treatment initiation, other vaccines within 2 weeks prior to study treatment initiation.
• •13. Serious illness or condition, which according to the Investigator poses an undue risk for the patient when participating in the trial, including, but not limited to, any of the following:
• •1. Clinically significant cardiovascular disease (myocardial infarction or stroke within last 6 months, uncontrolled angina within last 3 months, diagnosed or suspected clinically significant ventricular arrhythmias, ejection fraction \<35%, cerebrovascular event within last 6 months, uncontrolled hypertension \[blood pressure ≥180 mm Hg systolic and 110 mmHg diastolic despite medication\])
• •2. New York Heart Association Class III to IV congestive heart failure
• •3. Symptomatic peripheral vascular disease
• •4. Severe pulmonary disease (e.g., severe chronic obstructive pulmonary disease, pneumonitis or interstitial lung disease)
• •5. Uncontrolled diabetes (repeated episodes of severe hypo- or hyperglycemia requiring hospitalization)
• •6. Severe mental retardation/impairment, psychiatric conditions or substance abuse resulting in inability to understand informed consent or affecting the patient's cooperation in the study
• •7. Severe infection/inflammatory conditions
• •14. History of other malignancies (except for those which have been adequately treated and have had no recurrence).
• •15. Previous anaphylactic or severe allergic reaction to an LNP formulated drug or vaccine (e.g., Comirnaty or Spikevax) or known allergy to any other component of CVGBM (e.g., PEG).
• •16. Allergy to aminoglycoside antibiotics.
• •17. Pregnant or breastfeeding.
• •18. Prior (within 30 days prior to study enrollment) or concurrent participation in another interventional clinical trial studying an investigational product, drug or treatment regimen. At least 30 days should have passed prior to the first study treatment with the investigational product (exceptions may be considered on a case-by-case basis after consultation with the CureVac Medical Director).