Study of the Composition and Bacterial Diversity of the Vaginal Microbiota in Healthy Versus Pathological Conditions (Bacterial Vaginosis) Using a Targeted Metagenomic Approach (RNA 16s)

Inclusion Criteria

• •Woman aged between ≥ 18 and ≤ 45 years old,
• •In good general health (outside the gynaecological sphere), i.e. no chronic pathology and not taking medication at the time of inclusion and/or long-term,
• •Woman with a healthy or pathological vaginal flora according to Amsel's clinical diagnosis and Nugent's score :
• •Population with bacterial vaginosis (verification of at least the following 3 criteria + Nugent score):
• •Vaginal pH \> 4.5
• •greyish, homogenous vaginal secretions adhering to the vaginal wall
• •characteristic vaginal odour of "rotten fish" after contact of the vaginal discharge with a few drops of 10% potash ("sniff test")
• •Presence of "Clue Cells" in the microscopic examination of fresh vaginal secretions. Clue cells are cells of the ectocervix lined with gram-negative bacilli.
• •* Nugent Score ≥ 7
• •Healthy population (Verification of at least 3 following criteria + Nugent score) :
• •Absence of vaginal pH \> 4.5
• •No greyish, homogenous vaginal secretions adhering to the vaginal wall
• •No characteristic vaginal odour of "rotten fish" after contact of the vaginal discharge with a few drops of 10% potash ("sniff test").
• •Absence of "Clue Cells" on microscopic examination of fresh vaginal secretions. Clue cells" are cells of the ectocervix lined with gram-negative bacilli.
• •* Nugent Score ≤3
• •* Able and willing to participate in the research by complying with the procedures of the protocol, especially regarding vaginal sampling,
• •* Having given its free, informed and written consent.