Metabolic Profiling of Immune Responses in Immune-mediated Diseases

Study Description: This is an exploratory sample collection study enrolling adult healthy volunteers and patients with immune-mediated diseases to elucidate the metabolic signatures associated with immune-mediated diseases. We hypothesize that activation of, and responses to, specific immune pathways will require metabolic changes within cells, serum, and skin. Following screening and baseline procedures, including blood draw, participants who are able to attend onsite visits will undergo skin biopsies. The skin biopsies will involve up to 4 biopsies: 2 initial punch biopsies 2 mm in diameter, optionally followed by 1 or 2 punch biopsies 3 (+/-10) days later using a 3-mm punch to encompass the initial biopsy site(s), capturing the tissue at 3-14 days of healing. There will be an option for blister induction to maximize keratinocyte collection. Blister induction involves creating 8 blisters on the forearm and removing the tops of the blisters for primary cell culture derivation. In addition, skin swabs and tape strips may be collected to assess for microbial or host markers of epithelial repair. Peripheral blood and functional signatures will be collected with the initial and follow-up biopsies to compare metabolic and functional signatures in peripheral blood cells and serum with those in the skin. Urine may be collected in order to detect metabolites that are not as easily detected in the blood. After day 0 (or 3-14, if the participant has that optional visit), participation in the study will be complete. At-home participants who cannot attend onsite visits may enroll for survey data collection and optional at-home sample collection (with at least one sample type required). Exposome data will be collected to assess how environmental, occupational, and dietary exposures associate with the results. Samples and data will be stored for future research use. Objectives: Primary Objectives: 1. Evaluate metabolic profiles in immune activation associated with known or suspected immune-mediated disorders. 2. Determine whether there are abnormalities in specific tissue repair pathways, such as epithelial to mesenchymal transition (EMT), that are associated with immune-mediated disorders. Secondary Objectives: Not applicable. Exploratory Objective: Evaluate how metabolic profiles in immune activation vary with differing occupational, environmental, and/or dietary exposures. Endpoints: Primary Endpoints: 1. Fold differences in metabolic pathways associated with immune pathways. 2. Fold differences in metabolic pathways related to wound healing. 3. Change in relative abundance of microbial skin taxa and/or skin metabolites associated with wound healing or immune-mediated disorders. Secondary Endpoints: Not applicable. Exploratory Endpoints: 1. Association between fold differences in metabolic pathways associated with immune pathways and environmental, occupational, and/or dietary exposures. 2. Association between fold differences in metabolic pathways related to wound healing and environmental, occupational, and/or dietary exposures. 3. Association between change in relative abundance of microbial skin taxa and/or skin metabolites associated with wound healing or immune-mediated disorders and environmental, occupational, and/or dietary exposures.