Shivering and Glucose Homeostasis

Inclusion Criteria

• •Signed informed consent
• •Caucasian
• •Healthy (as determined by dependent physician based on medical questionnaire)
• •Man, or postmenopausal (defined as at least 1year post cessation of menses) woman
• •Aged 40 - 75 years at the start of the study
• •BMI 27 and 35 kg/m2
• •Stable dietary habits (no weight loss or gain \>3kg in the past 3 months)
• •Medication use known to hamper subject's safety during study procedures
• •Co-morbidities to which the intervention or program that may pose as a complicating factor
• •Subjects diagnosed with cardiovascular diseases or cancer
• •Fasting plasma glucose ≥ 7.0 mmol/l
• •Haemoglobin \< 7.8 mmol/l for women, or \< 8.4 mmol/l for men
• •Previously diagnosed with type 2 diabetes
• •Abuse alcohol/drug abuse
• •Subjects who do not want to be informed about unexpected medical findings during the screening/study or do not wish that their physician is informed.
• •Engagement in structured exercise \> 2h per week
• •Participation in another biomedical study within 1 month before the first study
• •Smoking
• •Cold-acclimated, such as takes daily extended cold baths, works in a refrigerated environment, or regular cold-water swimming within 1 month of starting the study