The Utility of Biofire Filmarray in Evaluation of Entero Pathogens Triggers in Patients With Chronic Diarrhea

Investigations: • Routine laboratory investigation: Complete blood picture and differential WBCs count, liver, kidney function tests, RBG, Na, K, CRP, ESR. • Microbilogical Investigations: To ensure that good specimens are provided for examination, it is important to note the following: * A sterile clean dry container must be used for the collection of fecal samples. * The specimen should be brought to the lab as soon as possible. * The specimen container should be clearly labeled with the patient's name, date, and time of passage of the specimen. A) Conventional methods: Stool samples will be cultured on Selenite broth then subcultured on blood agar, chocolate agar, MacConkeys agar, Sorbitol MacConkys agar: Xylose Lysine Deoxycholate agar (XLD) ,Sabouraud dextrose agar (SDA), blood agar with 10um/ml ampicillin , Cambylobacter CVA agar plates. 1. Identification of the bacterial organism: Pure colonies of isolated microorganisms were identified by: * Morphology on agar. * Gram stain film was made from the growth to identify morphology of the organism. * Biochemical reactions tests. 2. Detection of antibiotic sensitivity pattern according to CLSI 2019 by disc diffusion method 3. Confirmation of results by automated microbial system VITEK 2Compact. B) Multiplex PCR: for Identification of different causative organisms by Biofire microarray (BioMerieux,France)