Study to Evaluate the Safety, Tolerability, Pharmacokinetic, and Pharmacodynamic Effects of Repeat Topical Application of BOS-356 in Subjects With Moderate to Severe Acne Vulgaris

For Cohorts 1-5:

Participant is a female who is breastfeeding, pregnant, or who is planning to become pregnant during the study.

Participant has acne fulminans, conglobata, nodulocystic acne, or secondary acne.

Participant has a history of skin disease or presence of skin condition that, in the opinion of the investigator, would interfere with the study assessments.

Excessive facial hair that would interfere with diagnosis or assessment of acne vulgaris

Participant is known to have immune deficiency or is immunocompromised.

Participant has a history of cancer or lymphoproliferative disease within 5 years prior to Day 1. Participants with successfully treated nonmetastatic cutaneous squamous cell or basal cell carcinoma and/or localized carcinoma in situ of the cervix may be candidates for the study.

Participant had a major surgery within 8 weeks prior to Day 1 or has a major surgery planned during the study.

Participant has positive results for hepatitis B surface antigens (HBsAg), antibodies to hepatitis B core antigens (anti-HBc), hepatitis C virus (HCV), or human immunodeficiency virus (HIV).

Participant has used on the treated areas an over-the-counter (OTC) topical medication for the treatment of acne vulgaris, including benzoyl peroxide, topical anti-inflammatory medications, corticosteroids, salicylic acid, α-hydroxy/glycolic, or antibacterial/antiseptic soap or wash within 2 weeks prior to Day 1.

Participant has used prescription topical retinoid (e.g., tretinoin, tazarotene, adapalene), dapsone or antimicrobials (e.g., clindamycin, erythromycin), or other prescription topical medications for the treatment of acne vulgaris within 4 weeks prior to Day 1. Topical antibiotics may be used to treat non-acne skin lesions outside of the treated area.

Participant has used oral or injectable corticosteroids within 4 weeks prior to Day 1 or require them during the study. Note: Intranasal corticosteroids and inhaled corticosteroids are allowed. Eye and ear drops containing corticosteroids are also allowed.

Participant had a facial procedure (including, but not limited to, chemical peel, laser, microdermabrasion) within 8 weeks prior to Day 1 and during the study.

Participant has received photodynamic therapy or phototherapy within 12 weeks of Day 1.

Participant has used androgen receptor blockers (such as spironolactone or flutamide) within 12 weeks prior to Day 1.

Participant has used drospirenone, chlormadinone acetate, or cyproterone acetate within 26 weeks prior to Day 1.

Participant has used oral retinoid (e.g., isotretinoin) within 52 weeks prior to Day 1 or vitamin A supplements \>10,000 Units per day (U/d) within 26 weeks prior to Day 1.

Participant is currently receiving a nonbiological investigational product or device or has received one within 4 weeks or five half-lives (whichever is longer) prior to Day 1.

Participant has received blue light, laser, intense pulsed light or other light therapy on the face within 4 weeks prior to Day 1.

Participants who have given \> 50 milliliters (mL) of blood or plasma within 30 days of Screening or \> 499 mL of blood or plasma within 56 days of Screening (during a clinical trial or at a blood bank donation).

Participant has a known history of clinically significant drug or alcohol abuse in the last year prior to Day 1, or has a positive drug screen and/or positive alcohol test result at the Screening visit or at Day 1 (excluding cannabinoids).

Participant has had excessive sun exposure or has used tanning booths within 4 weeks prior to Day 1 or is not willing to minimize natural and artificial sunlight exposure during the study. Use of sunscreen products (except on treated areas) and protective apparel are recommended when sun exposure cannot be avoided.

For Cohorts 1-3 only:

Participant has a Fitzpatrick's Skin Phototype ≥5.

For Cohort 4 only, due to biopsy collection:

Participant has a history of an allergic reaction or significant sensitivity to lidocaine or other local anesthetics.

Participant has a history of hypertrophic scarring or keloid.

Participant has taken anticoagulant medication, such as heparin, low molecular weight (LMW)-heparin, warfarin, antiplatelets, within 2 weeks prior to Day 1, or has a contraindication to skin biopsies. Nonsteroidal anti-inflammatory drugs (NSAIDs) will not be considered antiplatelets and will be allowed.

What this study is about