Long-term, Substantial Weight Loss and Insulin Regulation of Lipolysis

Exclusion Criteria

• •Type 1 or Type 2 Diabetes diagnosis or fasting plasma glucose ≥126 mg/dL
• •Active coronary artery disease
• •Participation in structured exercise (\>2 times per week for 30 minutes or longer)
• •Smoking
• •Medications known to affect adipose tissue metabolism (e.g., beta blockers, corticosteroids)
• •Renal insufficiency (serum creatinine \> 1.5mg/dl)
• •Chronic active liver disease (Bilirubin \> 17mmol/L, AST \> 144 IU/L, or ALT\>165IU/L)
• •Pregnancy or breastfeeding.