Alveolar Macrophage Programming Following Endotoxin Exposure

Exclusion Criteria

• •1. Current or recent illness (past 2 weeks)
• •2. Presence or prior history of cardiac, pulmonary or systemic disease
• •3. Bleeding disorder, use of systemic anticoagulants or antiplatelet therapy
• •4. American Society of Anesthesiology (ASA) class 2 or greater
• •5. Immunocompromised state (HIV, immunoglobulin deficiency, systemic immunosuppressants)
• •6. Use of any inhaled substance, including tobacco, marijuana, e-cigarrettes, cocaine, methamphetamines, or toxic vapors in the past 3 months or greater than 10 pack-year smoking history
• •7. Alcohol use disorder or greater than 7 drinks/week for women or greater than 14 drinks/week for men in the past 3 months
• •8. Allergy or prior adverse reaction to lidocaine, midazolam or fentanyl
• •9. Abnormal spirometry or electrocardiogram at time of screening
• •10. Pregnant (based on urine pregnancy test) or breast feeding