Intravital Microscopy in Human Solid Tumors

PRIMARY OBJECTIVE: I. To determine the feasibility of performing human intravital microscopy (HIVM) in patients with deep space solid tumors during standard course of surgical resection. SECONDARY OBJECTIVES: I. Compare the microscopic observation of the tumor-associated vessels with normal tissue (e.g. peritoneal surface or normal brain tissue) in each individual subject. II. Correlate the microscopic observations of the tumor-associated vessels with pathologic grade of tumor. III. To correlate the microscopic observation of the microvasculature with tumor-specific and overall survival. OUTLINE: Patients receive fluorescein intravenously (IV) and undergo HIVM over 1-2 minutes per field. After completion of study, patients are followed up at 2-3 weeks after surgery.