Establishment of the 2H-labeling Protocol at Pennington Biomedical Research Center

Inclusion Criteria

• •Pre-menopausal women
• •18-40 years of age
• •BMI 25-29.9 kg/m2 (+/- 0.5 will be accepted)
• •Are willing to drink deuterium-labeled water (2H2O) for 6 weeks
• •Must be willing to comply with all study procedures, including adipose biopsies and a DXA scan
• •If applicable, hormonal contraception must have been started at least 2 months before entering the study. If enrolled, subject must also agree to not alter hormonal birth control method, dose, or regimen throughout the duration of the study
• •If not using pharmaceutical (hormonal) contraception (i.e. birth control pills, vaginal ring, injections, or implant), agree to use a double barrier method as a form of birth control to prevent pregnancy
• •Must be classified as either sedentary (achieve \< 75 minutes of moderate-intensity or \< 37 minutes of vigorous-intensity aerobic physical activity per week) or very physically active (achieve \> 225 minutes of moderate-intensity or \> 112 minutes of vigorous-intensity aerobic physical activity per week). Simple questions to assess physical activity level will be administered during the phone screening as well as the clinic screening visit.
• •If enrolled, agree to not alter dose, level, or regimen of physical activity throughout the duration of the study