Sedentary Behavior Interrupted: A Trial of Acute Effects on Biomarkers of Healthy Aging

Exclusion Criteria

• •Mental state that would preclude complete understanding of the protocol or compliance;
• •type 1 diabetes;
• •type 2 diabetes using insulin or with poor glycemic control (greater than 10% for all participants);
• •poorly controlled hypertension (Systolic Blood Pressure ≥165 or Diastolic Blood Pressure ≥100);
• •chronic illness that may be associated with weight change (HIV/AIDS, active cancer, or uncontrolled thyroid disease),
• •anemia (hemoglobin ≤11g/dL);
• •personal or first-degree relative history of venous thrombosis;
• •weight instability in past 3 months (no more than 5% up or down);
• •regular use of vasodilator medication and high risk of stroke and/or heart attack (i.e., history of multiple hospitalizations (\>2X) in the last 6 months), congestive heart failure, atrial fibrillation, and/or stroke;
• •regular use of immunosuppressant or corticosteroid medication;
• •blood donation less than 56 days prior to screening visit,
• •smoking cigarettes or smoking or ingesting anything, and other use of tobacco products including e-cigarettes.
• •diagnosis of orthostatic hypotension