PRIMARY OBJECTIVES:
I. Develop blood sample collection and preparation procedures for reliable, meaningful metabolomic profiling of peripheral blood mononuclear cells (PBMCs) by liquid chromatography/quadruple-time of flight/mass spectrometry (LC/Q-TOF/MS) that can be implemented in a clinical setting.
II. Optimize data analysis methods and software usage to create a metabolic profile for patients at the time of blood collection.
III. Compare metabolite profiles of isolated PBMCs versus (vs.) other blood fractions (plasma, red blood cells \[RBCs\], and whole blood).
IV. Identify elements of the PBMC metabolite profile that may correlate to patient disease state.
OUTLINE:
Patients undergo collection of blood for metabolic profiling via LC/Q-TOF/MS.
