Transfusion of Biotin-Labeled Red Blood Cells

Exclusion Criteria

• •Subjects with a past medical history or symptoms of blood dyscrasia, diabetes mellitus, hyperlipidemia, obstructive sleep apnea, renal disease, congestive heart failure, significant cardiac disease and / or known peripheral arterial disease.
• •Moderate to severe systemic hypertension (systolic blood pressure \>140 mmHg and/or diastolic blood pressure \> 95 mmHg
• •Systolic blood pressure \<100 mmHg and/or diastolic blood pressure \< 60 mmHg on the study day.
• •Positive Direct Antiglobulin Test
• •Consumption of biotin supplements or raw eggs within 30 days
• •Treatment with antibiotics in the week before initiating study participation to avoid suppression of erythropoiesis, which may accompany infection.
• •Blood loss in the previous 8 weeks due to epistaxis, trauma, hemoptysis, gastrointestinal bleeding, diagnostic phlebotomy (\> 30 ml)
• •Subjects who report tobacco or marijuana smoking within 6 months of study.
• •Cognitively impaired subjects, or institutionalized persons and subjects unable or unwilling to complete written informed consent
• •Subjects with a history of blood donation within the last 56 days.
• •Use of other investigational drugs/devices within 30 days of screening.
• •Subjects taking any medication for the treatment of diabetes including insulin
• •Females of childbearing potential who are pregnant or unwilling to undergo pregnancy testing; females with positive pregnancy testing on screening day will be excluded.
• •History of prior transfusion reaction to blood products.
• •Allergic reaction to biotin
• •Donors with naturally occurring antibodies against BioRBCs will be excluded from the study