Basophil Activation Test to Diagnose Food Allergy

Exclusion Criteria

• •1. Clinically significant chronic illness other than atopic diseases;
• •2. Previous history of severe life-threatening reaction to the suspected food with documented decrease in oxygen saturation (\<90%), hypotension (≥20% reduction in systolic blood pressure) and/or admission to intensive care;
• •3. Unwillingness to comply with study procedures, namely to undergo a diagnostic food challenge;
• •4. Contra-indication for diagnostic food challenge, namely:
• •* Uncontrolled atopic diseases (e.g. eczema, asthma, rhinitis);
• •* Chronic medical conditions that pose significant risk in the event of anaphylaxis or treatment of anaphylaxis (e.g. cardiac disease, severe lung disease, pregnancy, mastocytosis);
• •* Inability to discontinue medications that might interfere with assessment or safety (e.g. antihistamines, β-agonists, β-blockers, NSAIDs, ACE inhibitor, antacids);
• •* Recent (within 7-14 days) treatment with systemic steroids or prolonged high-dose systemic steroids or immunosuppressants;
• •5. Undergoing treatment with omalizumab, food allergen immunotherapy or other systemic immunomodulatory treatment;
• •6. Inability to stop anti-histamines prior to SPT.