A Chronological Study of the Formation of HIV Cellular Reservoirs Through the Expression of Surface Markers on CD4 + T Lymphocytes, Including CD32a

To analyze the HIV viral sequences present in the CD32 + CD4a + T lymphocytes of the patients who have participated in the ANRS 139 TRIO trial, always followed and in virological success, and carrying multi-resistant viruses archived in the HIV cell reservoir, in order to reconstruct the chronology of the installation of this reservoir.

Objectives Principal objective To analyze the HIV viral sequences present in the CD32 + CD4a + T lymphocytes of the patients who have participated in the ANRS 139 TRIO trial, always followed and in virological success, and carrying multi-resistant viruses archived in the HIV cell reservoir, in order to reconstruct the chronology of the installation of this reservoir. Secondary objectives To analyze the HIV viral sequences present in the CD4 + lymphocytes with other membrane HIV reservoir markers, currently being identified, in these same patients Methodology Pathophysiological study, in patients infected by multi drug-resistant viruses Estimated enrollment 21 participants (total and per group) Intervention 167mL blood sample in EDTA tube: * 160mL (16 tubes of 10mL): for IGH (Institute of Human Genetics-Montpellier) * 7mL (1tube of 7mL): to measure HIV-RNA in centers Estimated planning or Study / Trial timetable Study start date: September 2017 Enrollment period: 12 months Total study duration: 24 months (analyses included) Estimated study/trial completion date: September 2019 (one year after enrollment of the last patient) Study design Cross-sectional survey, 167mL of blood in EDTA tube will be obtained on a research visit, after signature of written informed consent