Serial Phlebotomy in Voluntary Blood Donors

Exclusion Criteria

• •known intolerance of phlebotomy procedures
• •major trauma or surgical procedures in the last 2 years
• •menstrual or other uterine bleeding in the last 2 years
• •chronic oral anticoagulation or dual antiplatelet therapy
• •chronic non-steroidal anti-inflammatory drug use
• •known history of hemochromatosis or other disorder of hematopoiesis or Fe metabolism
• •chronic oral Fe supplementation other than Fe-containing multivitamins
• •history of active cancer in the past 2 years
• •known history of chronic inflammatory disease
• •uncontrolled hypertension
• •electrocardiographic evidence of prior myocardial infarction
• •diabetes mellitus
• •fasting glucose \>100 mg/dL
• •body mass index \>40 kg/m2
• •any tobacco use in the past 6 months