The Human Microbiome in Immune-Mediated Diseases

Exclusion Criteria

• •Ongoing chronic infection (viral, bacterial or fungal) including known HIV, Hepatitis B/C
• •Acute infection receiving any antibiotics or any use of antibiotics within 90 days prior to screening
• •For skin swab collection (see also appendix D):
• •* No use of topical antibiotics within 7-days prior to collection of swab, other than use in normal hand washing.
• •* No use of topical antimicrobial products (as outlined in appendix F) within 48 hours prior to collection of swab
• •* Subject must not have bathed within 8-hours of swab collection.
• •For oral swab collection (see also appendix D):
• •* No use of antiseptic mouth washes (as outlined in appendix F) within 48 hours of swab collection
• •* Subjects must not have brushed teeth or flossed within 8-hours of swab collection
• •Major gastrointestinal surgery less than 5 years prior to enrollment (with the exception of appendectomy)
• •Any Gastrointestinal bleeding history
• •Inflammatory Bowel Disease diagnosed by biopsy
• •Bulimia or anorexia nervosa
• •Probiotics (greater than estimated 109 cfu or organisms per day) within 90 days prior to enrollment (with the exception of fermented beverages, milks or yogurts).
• •Morbid obesity (BMI ≥ 40)
• •Type I Diabetes Mellitus
• •Diabetes Mellitus type 2, poorly controlled defined as Hgb A1c greater than 8% on medical therapy
• •Malignancy within one year prior to screening (with the exception of non-metastatic squamous or basal cell skin carcinomas and cervical carcinoma if received curative surgical treatment)
• •Known illicit drug or alcohol abuse