Medication or supplements known to affect either endothelial function or lipid metabolism (i.e. arginine, protein, fish oil)
Acute illness
Uncontrolled metabolic disease, including liver or renal disease
Atrial fibrillation, history of syncope, limiting or unstable angina, congestive heart failure, known or suspected right-to-left, bi-directional or transient right-to-left cardiac shunts
Cardiac pacemaker or other medical device implanted in the body
Pulmonary hypertension or other unstable cardiopulmonary conditions
ECG documented abnormalities or valvular disease
Low hemoglobin or hematocrit (i.e., lower than accepted laboratory values)
History of hypertension or elevated blood pressure (systolic, \>140 mmHg or a diastolic, \>95 mmHg)
History of hyperlipidemia or plasma triglyceride concentration \>200 mg/dl
Diagnosed diabetes, or 2-h plasma glucose \>200 mg/dl during an oral glucose tolerance test
Current participation in a weight-loss regimen, including extreme dietary practices
Use of anabolic steroids or corticosteroids (within 3 months)