Impact of High-dose Folic Acid Supplementation on Pathways Linked to DNA Formation

Exclusion Criteria

• •Unable to swallow tablets
• •Unable to receive intravenous catheters
• •Use of vitamin supplements, amino acid or protein supplements for more than six months before study participation
• •Chronic consumption of a high-protein diet (e.g. Atkins Diet)
• •Medical conditions such as diabetes, asthma, cancer, cardiovascular disease, a history of neural tube defect (such as spina bifida) affected pregnancy, have had gastrointestinal surgery, abnormal kidney, thyroid function, or psychiatric illness, or any other chronic disease
• •Pregnancy or lactation
• •Smoking, use of recreational drugs, and/or consumption of more than one alcohol drink per day or more than seven per week.
• •Use of long-term prescription medication such as hormonal contraceptives, antidepressants, anticonvulsants, anticoagulants, or other chronic medication
• •Blood donation in the last three months prior to study start
• •Unable to provide informed consent, or unable to read and write English
• •Low compliance to intervention as determined by early steady-state or reduction in blood folate concentrations