Biological Standardization of D. Glomerata, L. Perenne, S. Cereale and O. Europaea Pollen Extracts

Inclusion Criteria

• •A. Subjects sensitized to one or more allergen extracts:
• •1. Positive clinical history with inhalant allergy to at least one of the allergen to be standardized.
• •2. At least one positive prick test (mean wheal diameter greater or equal than 3mm) .
• •3. Positive prick test (wheal diameter greater or equal to 3 mm) to Histamine 10 mg/ml
• •4. Age: 18-60 years
• •5. Written informed consent
• •6. Patients will preferably be monosensitized, or with clinically relevant sensitization , or with primary sensitization (maximum cutaneous reactivity) to the extracts under investigation.
• •7. Women of child-bearing potential must have a negative urine pregnancy test at the time they begin the study.
• •B. Atopic Subjects:
• •1. Age: 18-60 years
• •2. Written informed consent
• •3. Positive prick test (wheal diameter greater or equal to 3 mm) to Histamine 10 mg/ml
• •4. Negative prick test (mean wheal diameter less or equal to 3mm) when tested with already standardized extracts of the allergens under investigation or with cross reactive extracts.
• •5. Women of child-bearing potential must have a negative urine pregnancy test at the time they begin the study.
• •C. No Atopic Subjects:
• •1. Age: 18-60 years.
• •2. Written informed consent
• •3. Positive prick test (wheal diameter greater or equal to 3 mm) to Histamine 10 mg/ml
• •4. Negative prick test (mean wheal diameter less or equal to 3mm)to all allergens tested in diagnostic prick test.
• •5. Women of child-bearing potential must have a negative urine pregnancy test at the time they begin the study.