Circulating Tumor Cells and Melanoma: Comparing the EPISPOT and CellSearch Techniques

The secondary objectives of this study include: A. To compare the following elements between the two patient groups: * the number of CMCs per ml of blood as determined by EPISPOT * the number of CMCs per ml of blood as determined by CellSearch * the percentage of patients with at least 2 CMCs per ml of blood according to the two techniques * the % of CMCs expressing KI67 * the % of CMCs expressions S100 (only the EPISPOT technique) B. To compare the EPISPOT and CellSearch techniques is terms of the following: * the number of CMCs detected per ml blood * the number of CMCs expressing antigen KI67 C. To re-evaluate the 2-CMC per ml blood threshold