Neurogenic Inflammation in Diabetes

Exclusion Criteria

• •Peripheral arterial disease: toe pressure \< 70 mm Hg and/or transcutaneous oxygen tension \< 40 mm Hg and/or claudication.
• •Renal insufficiency: MDRD creatinin clearance \< 30 ml/min.
• •Systemic disease such as vasculitis or rheumatoid arthritis.
• •Malignancy.
• •(Diabetic) foot ulcer.
• •Gout.
• •Bacterial infection of an extremity.
• •Skin condition of the dorsal aspect of the foot or the medial side of the upper arm.
• •Bleeding disorder such as hemophilia.
• •Use of medication for asthma.
• •Impaired immunity such as in HIV/AIDS.
• •Capillary blood glucose \< 3 mmol/l or \> 20 mmol/l at the time of the study.
• •Peripheral oedema.
• •Vaccination in the two months prior to study inclusion.
• •Chemotherapy or radiation therapy in the twelve months prior to study inclusion.
• •Surgery in the two months prior to study inclusion.
• •Previous adverse reaction to Candida albicans antigen.
• •Acute infection at the time of the study or in the month prior to study inclusion.
• •Transfusion in the two months prior to study inclusion.
• •Use of immunosuppressants in the two months prior to study inclusion.
• •Pregnancy or breastfeeding.