FFT, Inflammation, Lipid Metabolism, Blood Pressure and Organ Damage in Patients With Obesity, Chronic Kidney Disease (CKD).

The study will be performed in two experimental groups. Proposed study groups Study I Patients with BMI\> 30 and metabolic syndrome. Patient with BMI\> 30 without metabolic syndrome. Normal healthy controls. Study II Patients with CKD stage III and uric acid \< 7 mg/dl Patients with CKD stage III and uric acid \> 7 mg/dl Patient with asymptomatic hyperuricemia and eGFR \> 90 ml/min/1.73 m2, , uric acid \> 7 mg/dl Hemodialysis patients Characteristics of the particular patients group- including criteria: 10- 15 participants in each subset of each group age 18-65 y. Gender: females and males in equal proportion. Group I Patients with BMI\> 30 and metabolic syndrome ( ATP III). Patient with BMI\> 30 without metabolic syndrome. Normal healthy control- healthy persons( without renal disease, cardiovascular diseases, diabetes mellitus, BMI \< 25;normotensives ). Group II Patients with CKD stage III (GFR 30-59 ml/min/1,73 m2) and uric acid \< 7 mg/dl. * without diabetes mellitus proteinuria \< 3,5 g/24 h without immunosuppressive agents, ACEi, ARB, allopurinol treatment well controlled hypertension ( \< 140/90 mmHg) Patients with CKD stage III(GFR 30-59 ml/min/1,73 m2) and uric acid \> 7 mg/dl * without diabetes mellitus proteinuria \< 3,5 g/24 h without immunosuppressive agents, ACEi, ARB, allopurinol treatment well controlled hypertension ( \< 140/90 mmHg) Patient with asymptomatic hyperuricemia, uric acid \> 7 mg/dl with normal renal function Hemodialysis patients. * CKD: nondiabetic nephropathy * duration hemodialysis 3-48 months * Hb-11-13 g/dl * well controlled hypertension ( \< 140/90 mmHg) * without ACEi, ARB, allopurinol treatment * residual diuresis will be estimated for last 48 hours-between mid and next dialysis Method of investigation All patients and controls ( but hemodialysis) will present after overnight fast. Baseline tests include 24 hr urine collection for: sodium, calcium, phosphorus, creatinine, uric acid, NAG, albumin will be carry out and the same morning fasting sample of blood for: creatinine, cystatin C , uric acid, sodium, glucose, insulin, triglycerides, HDL and LDL cholesterol, calcium, phosphorus, hsCRP. BP will be determined as the mean of three readings taken 5 min apart while sitting. Next in all patients and controls fructose tolerance test will be performed. The test consists of giving 1 gram/kg b.w. of fructose p.o. with blood collection at 0,30,60 and 120 min afterward for serum uric acid determination and uric acid area under the curve will be calculated. The calculated area under curve is the measure of occult uric acid disturbance when compared to controls. Day before the urine collection the ABPM , BMI, IM (intima media ratio) , renal duplex ultrasound (RI) will be done Measure BMI, waist circumference