APSV in Vaccinia Naive Adults

Exclusion Criteria

• •Allergies to any component of the vaccines (e.g., polymyxin B sulfate, dihydrostreptomycin sulfate, chlorotetracycline hydrochloride).
• •History of immunodeficiency.
• •Serology positive for HIV, hepatitis B, or hepatitis C at the screening visit.
• •Known or suspected impairment of immunologic function including, but not limited to clinically significant liver disease.
• •Diabetes mellitus.
• •Moderate to severe kidney impairment.
• •Malignancy with the exception of squamous cell or basal cell skin cancer.
• •Autoimmune disease.
• •Use of immunosuppressive medication.
• •Corticosteroid nasal sprays are permissible.
• •Medical or psychiatric condition or occupational responsibilities which preclude subject compliance with the protocol.
• •History of illegal injection drug use.
• •Live attenuated vaccines within 60 days of study.
• •Use of experimental agents within 30 days prior to study.
• •Any history of vaccination with any vaccinia vectored or other pox vectored experimental vaccine.
• •Smallpox vaccination since 1990 (Vaccinia experience cohorts).
• •Typical vaccinia scar or history of smallpox vaccination (Vaccinia naive cohorts only).
• •Receipt of blood products or immunoglobulin in the past 6 months.
• •Recent donation of the equivalent of a pint of blood within the 30 days prior to enrollment.
• •Acute febrile illness (100.5 F) on the day of vaccination.
• •Pregnant or lactating women.
• •Eczema of any degree or history of eczema.
• •History of exfoliative skin disorders/conditions.
• •Any acute skin disorders of large magnitude, e.g., laceration requiring sutures, burn greater than 2x2 cm.
• •Household contacts/sexual contacts with, or close and frequent occupational exposure to, any of the following: pregnant women, children \< 12 months of age, people with eczema or history of eczema, people with any of the skin disorders/conditions previously listed, people with immunodeficiency disease or use of immunosuppressive medications.
• •Any condition which, in the opinion of the investigator, might interfere with study objectives.