Human Immune Responses Smallpox

Exclusion Criteria

• •History of immunodeficiency
• •Known or suspected impairment of immunologic function including but not limited to clinically significant liver disease, diabetes mellitus, moderate to severe kidney impairment
• •Malignancy other than squamous cell or basal cell skin cancer
• •Autoimmune disease
• •Live attenuated vaccines within 60 days of study
• •Known allergies to any component of the vaccine (e.g. polymyxin B sulfate, dihydrostreptomycin sulfate, chlortetracycline hydrochloride, neomycin sulfate)
• •Known allergies to any known component of VIG i.e. thimerosal or previous allergic reaction to immunoglobulins
• •Acute febrile illness on the day of vaccination
• •Eczema of any degree or history of eczema
• •Use of immunosuppressive medication. Corticosteriod nasal sprays are permissible
• •Medical or psychiatric condition or occupational responsibilities that preclude subject compliance with the protocol
• •History of "illegal" injection drug use
• •Inactivated vaccine 14 days prior to vaccination
• •Use of experimental agents within 30 days prior to study
• •Receipt of blood products or immunoglobulin in the past 6 months
• •Pregnant or lactating women
• •History of chronic exfoliative skin disorders/conditions
• •Any acute skin disorders of large magnitude e.g. laceration requiring sutures, burn greater than 2 x 2 cm
• •Any condition that, in the opinion of the investigator, might interfere with study objectives
• •Known allergies to any known component of the diluent (i.e. glycerin and phenol)
• •Known allergies to cidofovir or probenecid
• •Household contacts/sexual contacts with, or occupational exposure to (other than minimal contact), any of the following: 1) pregnant women, 2) children less than 12 months of age, 3) people with a history of eczema, 4) people with chronic exfoliative skin disorders/conditions or any acute skin disorders of large magnitude e.g. laceration requiring sutures, burn greater than 2 x 2 cm, or 5) people with immunodeficiency disease or use of immunosuppressive medications